[1]方海仙,梅文泉,杨东顺,等.紫外分光光度法快速测定山葵中异硫氰酸酯的研究[J].调味品杂志(月刊),2016,(10):109-111.[doi:10.3969/j.issn.1000-9973.2016.10.024]
　FANG Hai-xian,MEI Wen-quan,et al.Study on Rapid Determination of Isothiocyanates in Wasabia japonica Mastum by Ultraviolet Spectrometry[J].CHINA CONDIMENT,2016,(10):109-111.[doi:10.3969/j.issn.1000-9973.2016.10.024]
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紫外分光光度法快速测定山葵中异硫氰酸酯的研究
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调味品杂志（月刊）[ISSN:1000-9973/CN:23-1299/TS]

卷:
期数:
2016年10期
页码:
109-111
栏目:
分析检测
出版日期:
1900-01-01

文章信息/Info

Title:
Study on Rapid Determination of Isothiocyanates in Wasabia japonica Mastum by Ultraviolet Spectrometry
文章编号:
1000-9973(2016)10-0109-03
作者:
方海仙1; 2; 梅文泉1; 2; 杨东顺1; 2; 汪禄祥1; 2*
1.云南省农业科学院质量标准与检测技术研究所,昆明 650223;
2.农业部农产品质量监督检验测试中心,昆明 650223
Author(s):
FANG Hai-xian1;  2;  MEI Wen-quan1;  2;  YANG Dong-shun1;  2;  WANG Lu-xiang1;  2*
1.Agri-Food Quality Standard and Testing Technology Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China;
2.Supervision & Testing Center for Farm Products Quality, Ministry of Agriculture, Kunming 650223, China
关键词:
山葵;  异硫氰酸酯;  紫外分光光度法;  快速测定
Keywords:
Wasabi japonica Mastum;  isothiocyanates;  ultraviolet spectrometry;  rapid determination
分类号:
TS201.2
DOI:
10.3969/j.issn.1000-9973.2016.10.024
摘要:
目的:建立一种快速测定山葵中异硫氰酸酯含量的方法。方法:采用紫外分光光度法,以烯丙基异硫氰酸酯为对照品,测定249 nm处的吸光度。结果:对照品在0～80 μg/mL范围内,吸光度呈良好的线性关系(R=0.9997),回归方程为Y=0.0127X-0.013,加标回收率在86.7%～98.8%之间,方法相对标准偏差为1.09%～3.33%,测得山葵中异硫氰酸酯含量为0.079%。结论:该方法简单快速、准确度高、重复性好,为进一步开发利用山葵提供了理论基础。
Abstract:
Objective: A method for the rapid quantification of isothiocyanates in Wasabi japonica Mastum is established. Methods: In this test, the content of isothiocyanates is detected by ultraviolet spectrometry, allyl isothiocyanate is regarded as the standard, and the maximum absorption wavelength is at 249 nm. Results: The good linearity between concentration and absorbance of the standard is within the range of 0～80 μg/mL with a relativity coefficient of 0.9997, the linear regression equation is: Y=0.0127 X-0.013. The recovery rate is 86.7%～98.8% and the relative standard deviation is 1.09%～3.33%, the isothiocyanates content in Wasabi japonica Mastum is 0.079%. Conclusions: The method is simple and fast, has high accuracy and good repeatability, and it has provided theoretical basis for the further development and utilization of Wasabi japonica Mastum.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:方海仙(1988-),女,云南师宗人,助理研究员,硕士,主要从事食品安全检测工作。
更新日期/Last Update: 1900-01-01