[1]黄巍,等.香旱芹精油对金黄色葡萄球菌抑菌机理的研究[J].中国调味品,2017,(12):7-11.
　HUANG Wei,WANG Jian-qing,ZHANG Qian.Study on the Antibacterial Mechanism of Ajowan Essential Oil Against Staphylococcus aureus[J].CHINA CONDIMENT,2017,(12):7-11.
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香旱芹精油对金黄色葡萄球菌抑菌机理的研究()
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《中国调味品》[ISSN:1000-9973/CN:23-1299/TS]

卷:
期数:
2017年12期
页码:
7-11
栏目:
出版日期:
2017-12-01

文章信息/Info

Title:
Study on the Antibacterial Mechanism of Ajowan Essential Oil Against Staphylococcus aureus
作者:
黄巍1;  2; 王建清1; 张倩1
(1.天津科技大学中国轻工业食品包装材料与技术重点实验室,天津 300222 ;2. 中国包装科研测试中心,天津 300457 )
Author(s):
HUANG Wei 1; 2;  WANG Jian-qing 1 ;  ZHANG Qian 1
(1.Tianjin University of Science and TechnologyChina Light Industry Key Laboratory of Food Packaging Materials and Technology, Tianjin 300222,China; 2.China Packaging Research & Test Center, Tianjin 300457, China)
关键词:
香旱芹精油; 微波辅助水蒸气蒸馏; GC-MS分析; 金黄色葡萄球菌; 抑菌机理
Keywords:
Ajowan essential oil;  microwave assisted steam distillation;  GC-MS analysis;  Staphylococcus aureus;  antibacterial mechanism
分类号:
TS201.3
文献标志码:
A
摘要:
采用微波辅助水蒸气蒸馏提取出香旱芹精油，对其成分进行GC-MS分析，通过测试其对金黄色葡萄球菌的抑菌圈直径、最低抑菌浓度( MIC) 和最低杀菌浓度( MBC) 评价其抑菌效果。分别从菌体微观形态，细胞溶出物、蛋白质和钾离子泄漏等方面研究香旱芹精油对金黄色葡萄球菌的抑菌机理。实验结果表明香旱芹精油的主要成分为百里香酚（45.85%），对伞花烃（20.72%）和γ-松油烯（23.57%），其对金黄色葡萄球菌的抑菌圈直径为75mm，MIC为0.25μL/mL，MBC为0.5μL/mL。香旱芹精油可使金黄色葡萄球菌细胞膜受到损伤，使细胞膜表面出现塌陷和破裂，并引起菌体细胞溶出物、蛋白质、以及钾离子的泄露，从而导致了菌体的死亡。
Abstract:
Ajowan essential oil is extracted by microwave assisted steam distillation, and its chemical composition is analyzed by GC-MS. The inhibitory diameter, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ajowan essential oil against Staphylococcus aureus are tested by agar diffusion method and dilution method.The antibacterial mechanism of ajowan essential oil against S.aureus is studied by exploring the scanning electron microscopy (SEM) image of S.aureus, the leakage of cell constituents, protein and potassium ion from S.aureus cell after suffering the treated with the ajowan essential oil. The results show that the main components of ajowan essential oil are thymol (45.85%) , p-cymene ( 20.72%),and γ-Terpinene(23.57%), which have a strong inhibitory effect on S. aureus, the diameter of the inhibition zone is 75 mm, the MIC is 0.25 μL/mL and the MBC is 0.5 μL/mL. Ajowan essential oil can destroy the cell membrane of S. aureus, and its cell membrane surface appeared folds and local collapse, resulting in a leakage of cell constituents, protein, and potassium ion from cell, leading to the death of bacteria

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更新日期/Last Update: 2017-12-04