[1]李玲陈凯 王贻莲 张薇杨玉忠扈进冬 李纪顺*.高产Monacolin K红曲菌种的筛选及发酵条件的优化[J].中国调味品,2017,(12):89-93.
　LI Ling,CHEN Kai,WANG Yi-lian,et al.LI Ling1, CHEN Kai1, WANG Yi-lian1, ZHANG Wei2, Yang Yu-zhong1, HU Jin-dong1, LI Ji-shun1*[J].CHINA CONDIMENT,2017,(12):89-93.
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高产Monacolin K红曲菌种的筛选及发酵条件的优化()
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《中国调味品》[ISSN:1000-9973/CN:23-1299/TS]

卷:
期数:
2017年12期
页码:
89-93
栏目:
出版日期:
2017-12-01

文章信息/Info

Title:
LI Ling1, CHEN Kai1, WANG Yi-lian1, ZHANG Wei2, Yang Yu-zhong1, HU Jin-dong1, LI Ji-shun1*
作者:
李玲陈凯1 王贻莲1 张薇2杨玉忠1扈进冬1 李纪顺1*
(1.山东省科学院生态研究所，山东省应用微生物重点实验室，济南250014； 2.农业部农药检定所,北京 100026)
Author(s):
LI Ling1;  CHEN Kai1;  WANG Yi-lian1;  ZHANG Wei2;  Yang Yu-zhong1;  HU Jin-dong1;  LI Ji-shun1*
(1.Ecology Institute of Shandong Academy of Sciences, Shandong Provincial Key Lab for Applied Microbiology, Jinan 250014, China; 2. Institute for the Control of AgrochemicalsChina)
关键词:
曲霉Monacolin K; N+离子注入诱变育种发酵条件
Keywords:
Monascus;  Monacolin K;  N+ ion implantation;  mutation breeding;  fermentation conditions
文献标志码:
Monascus w
摘要:
为了提高Monacolin K的产量，应用30 KeV的N+离子注入，对紫红曲霉M2 进行诱变育种。诱变剂量分别为1 × 1015、5 × 1015、1 × 1016、5 × 1016、1× 1017 N+ /cm2，通过构巢曲霉对峙培养、洛伐他汀抗性筛选、高效液相色谱HPLC方法筛选高产Monacolin K的优秀突变菌株，并且通过液态发酵试验优化部分培养条件。结果显示：相对原菌株M2，最佳诱变菌株11-13产Monacolin K的能力是原菌株M2的2.3倍，连续传代5次，其高产遗传形状稳定。其最佳发酵培养条件为：温度30 ℃，接种量10%，转速200 r/min，发酵时间14 。
Abstract:
In order to improve the yield of Monacolin K, Monascus purpureus M2 is mutagenized by 30 keV N+ ion implantation. The dose are 1 × 1015, 5 × 1015, 1 × 1016, 5 × 1016, 1× 1017 N+/cm2. Excellent mutant strains are obtained through the screening method of plate confrontation culture of Aspergillus nidulans, Monacolin K resistance and high performance liquid chromatography (HPLC). And the fermentation conditions are optimized by liquid fermentation experiments. Results show that the Monacolin K yield of the best mutant 11-13 is 2.3 times higher than initial strains M2. It remains steady after 5 generation of serial cultivation. The optimum liquid fermentation conditions of 11-13 are as follows：the temperature is 30 ℃, the volume of inoculums s 10%, the rotating speed is 200 r/min, the fermentation time is 14 days

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备注/Memo

备注/Memo:
山东省科学院青年基金项目(2017QN009) 资助；山东省重点研发计划（2016GNC113009）作者简介：李玲（1985—），助理研究员研究方向：应用与环境微生物李纪顺，高级工程师，研究方向:应用与环境微生物。
更新日期/Last Update: 2017-12-04