[1]连战,吕志飞,刘彬,等.碱法预处理麦糟酶解转化还原糖工艺研究[J].中国调味品,2018,(7):6-11.[doi:10.3969/j.issn.1000-9973.2018.07.002]
 LIAN Zhan,LV Zhi-fei,LIU Bin,et al.Study on the Enzymatic Hydrolysis of Alkaline Hydrogen Peroxide Pretreated Brewers Spent Grain (BSG) to Reducing Sugar[J].CHINA CONDIMENT,2018,(7):6-11.[doi:10.3969/j.issn.1000-9973.2018.07.002]
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碱法预处理麦糟酶解转化还原糖工艺研究(/HTML)
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《中国调味品》[ISSN:1000-9973/CN:23-1299/TS]

卷:
期数:
2018年7期
页码:
6-11
栏目:
出版日期:
2018-07-01

文章信息/Info

Title:
Study on the Enzymatic Hydrolysis of Alkaline Hydrogen Peroxide Pretreated Brewers Spent Grain (BSG) to Reducing Sugar
文章编号:
1000-9973(2018)07-0006-06
作者:
连战吕志飞刘彬刘欣庄倩倩刘同军
齐鲁工业大学(山东省科学院)
Author(s):
LIAN ZhanLV Zhi-feiLIU BinLIU Xin ZHUANG Qian-qianLIU Tong-jun*
(School of BioengineeringQilu University of Technology(Shandong Academy of Sciences, Jinan 250353, China)
关键词:
麦糟AHP预处理酶解糖转化率还原糖
Keywords:
brewers spent grain(BSG)alkaline hydrogen peroxide(AHP) pretreatmentsugar conversion
分类号:
TS201.1
DOI:
10.3969/j.issn.1000-9973.2018.07.002
文献标志码:
A
摘要:
研究通过NREL法分析麦糟成分,确定了纤维素酶与半纤维素酶添加量。单因素实验明确碱过氧化氢法(AHP)预处理NaOH的适宜添加量,比较了未预处理和AHP预处理的麦糟酶解效果。结果显示,最适NaOH添加量为0.08 g/g底物,AHP预处理能有效提高麦糟酶解糖得率;在20%底物浓度下,AHP预处理组葡萄糖与木糖转化率分别提高了29.8%,13.3%。在AHP预处理基础上对25%底物浓度麦糟水洗,结果表明,水洗能有效提高麦糟酶解糖转化率,较未水洗组,葡萄糖与木糖转化率分别提升8.23%和7.98%。在水洗基础上利用Design Expert进行Mixture实验设计,设计纤维素酶、半纤维素酶与果胶酶联合酶解麦糟的加酶比例。模型显示,最佳加酶比例为纤维素酶:半纤维素酶=0.63:0.37。在该加酶比例下,葡萄糖转化率达到89.73%,较优化前提升7.1%,木糖转化率也相对提升7.5%。
Abstract:
The major components of Brewers’ Spent Grain (BSG) re analyzed. Single factor experiment is conducted to optimize the amount of NaOH added during AHP pretreatment, the enzymatic hydrolysis of BSG with/without AHP pretreatment is also investigated. The result show that the proper addition of NaOH is 0.08 g/g, and AHP pretreatment could improve the sugar conversion of BSG efficiently compared with the untreated group . Under the biomass-loading of 20%, the glucose and xylose conversion of AHP pretreated group improve 29.8% and 13.3% respectively. The optimal time of enzymaitic hydrolysis is 24 h. The water wash of 25% biomass loading BSG based on the AHP pretreatment is performed, the results show that water wash could improve the sugar conversion, the glucose and xylose conversion improve 8.23% and 7.98% respectively comparing with the control group. The cocktail of Ctec2 ,Htec2 and pectinase is studied by using mixture design with the software Design Expert. The model shows that the optimum addition is ctec2:htec ~0.63:0.37 on the protein amount basis.The glucose conversion rate s 89.73% during enzymatic hydrolysis of water –washed BSG under this condition, which is increased by 7.1%, the xylose conversion has also improved 7.5%

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备注/Memo

备注/Memo:
基金项目:山东省自然科学基金(ZR2014BM031);山东省科技发展计划(2015GSF121022) 山东省自然科学基金博士基金(ZR2016CB03)作者简介:连战(1992-),男,硕士研究生,研究方向:生物制药; *通讯作者:刘同军(1971-),副教授,博士,硕士生导师,
更新日期/Last Update: 2018-08-08